产品货号：
Z30957
中文名称：
PERK/EIF2AK3抗体
英文名称：
Anti-PERK/EIF2AK3 Antibody
产品规格：
100μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-PERK/EIF2AK3 Antibody |
| 指标别名 | EIF2AK3;HRI;HsPEK;Pancreatic eIF2 alpha kinase;PEK;PERK;PERK/EIF2AK3;WRS |
| 克隆性 | Polyclonal |
| 检验物种 | mouse |
| 应用范围 | IHC,IP,WB |
| 基因名称 | EIF2AK3 |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | This whole rabbit serum was prepared by repeated immunizations with a recombinant fusion protein from amino acids 601-1115 of mouse deltaN PERK. |
| 计算分子量 | 125kDa |
| 成分 | 0.02M Potassium Phosphate,0.15M Sodium Chloride,pH7.2,0.01% (w/v) Sodium Azide |
| 纯化方式 | This antiserum is directed against PERK and reacts with the PERK from mouse tissues.Reactivity to other species is unknown. |
| 浓度 | 80mg/mL by Refractometry |
| 产品形态 | 溶液 |
| 保存条件 | Store vial at -20℃ prior to opening.Aliquot contents and freeze at -20℃ or below for extended storage.Avoid cycles of freezing and thawing.Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4℃ as an undiluted liquid.Dilute only prior to immediate use.Expiration date is one (1) year from date of opening. |
| 背景资料 | The PKR-like endoplasmic reticulum kinase (PERK,also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER).PERK consists of an N-terminal ER luminal domain,a membrane-spanning region,and a cytosolic C-terminal serine/threonine kinase domain (1).The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2).PERK activation occurs upon accumulation of misfolded proteins and the ER lumen,which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3,4).PERK phosphorylates two established targets:the eukaryotic translation initiation factor 2 alpha (eIF2,(1)) and the Nrf2 transcription factor (5).Phosphorylation of eIF2 results in attenuation of translation initiation (6).The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein,cyclin D1 (7).PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8).Thus,while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival. |
| Uniprot ID | Q9Z2B5 |
| 基因名全称 | Eukaryotic translation initiation factor 2-alpha kinase 3 |
| 推荐稀释比 | ELISA:1:4,000 - 1:20,000 IHC:1:1,000 IF Microscopy:User Optimized IP:10～30μL WB:1:500 - 1:3000 |
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